Prepare for aging procedure

 

Once the intellectual decision is taken, what next. Improvement by TPE (Therapeutic plasma exchange) means some questions. Consistent with our observations. This work improves our understanding of the systemic paradigms of multi-tissue rejuvenation and suggest a novel and immediate use of the FDA approved TPE for improving the health and resilience of older people.

Serious understanding on what exact risks and benefits are?

In exact, I found Conboy interview, essential reading to exact understanding of my belief. Starting 2001, I have chased SENS, of Dr Audrey de Grey. Then he was noticed as a target of MIT Kids after he claimed to be working on Long health span of 1000 years. I learned that he was no light weight, but medical head at Oxford. Computer Scientist who had changed to medicine. Then he was offering a million bucks for lengthening mouse life by 20% or more. I tracked him 20 years. His wife died (he was 10 yr younger), retired from Oxford, moved to the USA. SENS remains, his baby now, and he collects money and thus funds lifespan research over the world. In 2005, he funded doctor pair Irina and Michael at UC Berkeley, and they did the original parabiosis work and paper. He also funded the pairs work in 2020 which also include Mehdipour siblings to watch out in future. Almost every one else interpreted the original work to imply magic proteins in young blood and several doctors from Stanford and UCB formed companies to sell the magic proteins isolated from young blood and medicalized by them. Not Conboy couple who worked on alternate dilution interpretation and proved it Nov, 2020! Other than Dr. Sinclair, only Dr. de Grey gets my full attention re age. They have met and respect each other!

Listing of goals?

Health span. 20 years bioage subtraction. Best safety I can muster. Relief of non-fixable-allopathy diseases. Horvath age post procedure.

Who will be my seconds in TPE?

My father were six brothers and 2 sisters. That is 32 cousins, 12 from mother side. Several became doctors. Four maternal nieces are MD. My ex-wife was MD. She might be tempted to watch me suffer!

Deeper looks at aging?

In fact, a decade ago, a group of prominent scientists penned an open letter describing the SENS strategy as pseudoscience. Say what you will about Aubrey de Grey, but one of his greatest strengths is to challenge conventional thinking. All innovative ideas appear to be lunacy at some point. While Aubrey’s seven strategies of SENS may not all bear fruit, Dr. de Grey is overcoming the inertia that has long kept longevity science subdued. For ex. senolytic drugs have been developed and are in clinical use!

Rejuvenation of three germ layers tissues by exchanging old blood plasma with saline-albumin

Heterochronic blood sharing rejuvenates old tissues, and most of the studies on how this works focus on young plasma, its fractions, and a few youthful systemic candidates. However, it was not formally established that young blood is necessary for this multi-tissue rejuvenation. Here, using our recently developed small animal blood exchange process, we replaced half of the plasma in mice with saline containing 5% albumin (terming it a “neutral” age blood exchange, NBE) thus diluting the plasma factors and replenishing the albumin that would be diminished if only saline was used. Our data demonstrate that a single NBE suffices to meet or exceed the rejuvenative effects of enhancing muscle repair, reducing liver adiposity and fibrosis, and increasing hippocampal neurogenesis in old mice, all the key outcomes seen after blood heterochronicity. Comparative proteomic analysis on serum from NBE, and from a similar human clinical procedure of therapeutic plasma exchange (TPE), revealed a molecular re-setting of the systemic signaling milieu, interestingly, elevating the levels of some proteins, which broadly coordinate tissue maintenance and repair and promote immune responses. Moreover, a single TPE yielded functional blood rejuvenation, abrogating the typical old serum inhibition of progenitor cell proliferation. Ectopically added albumin does not seem to be the sole determinant of such rejuvenation, and levels of albumin do not decrease with age nor are increased by NBE/TPE. A model of action (supported by a large body of published data) is that significant dilution of autoregulatory proteins that crosstalk to multiple signaling pathways (with their own feedback loops) would, through changes in gene expression, have long-lasting molecular and functional effects.