Motivate this read
A question every intelligent reader should ask: why is theory of Information right, conflicting with EVERY idiot of the past. Why is arun arya digilog right and why does it exist? Correctness of theory of information is discussed here. Digilog is a strict subset and is right if extended theory is. It exists because the purpose of this research has nothing to do with Ageing, rather amelioration of ageing for long healthy life. Dr. Sinclair recommends things which will add 20-30 years of healthy life. There are other things in works which will add another 20-30 years and 50 years even enable thousand years! Time enough to reach wish-death. Remember Thinfo; earthian DNA has exclusion of reproduction and repair in DNA. Nothing is lost in DNA with age, it is all in the epigenome. An epi-clock can reliably predict age remaining - every moron's idea of age-extension can be numerically tested!
Proof experiment
To prove that [Theory of Information], we needed to break some mouse DNA. [easy by shear, chemo, x-ray etc] [precise] To do that, we got our hands on a gene similar to Cas9, the CRISPR gene-editing tool. from a gooey yellow slime mold called Physarumpolycephalum, which literally means “many-headed slime.” Most scientists believe that this gene, called I-PpoI, is a parasite that serves only to copy itself. When it cuts the slime mold genome, another copy of I-PpoI is inserted. It is the epitome of a selfish gene. cuts DNA at just a few places in the mouse genome, and there is no copying process. [I-PpoI cuts at random] Instead, the cell has no problem pasting the DNA strands back together, leaving no mutations, which is exactly what we were looking for to engage the survival circuit and distract the sirtuins.
To create a mouse to test the information theory, we inserted I-PpoI into a circular DNA molecule called a plasmid, along with all the regulatory DNA elements needed to control the gene, and then inserted that DNA into the genome of a mouse embryonic stem cell line we were culturing in plastic dishes in the lab. We then injected the genetically modified stem cells into a 90-cell mouse embryo called a blastocyst, implanted it into a female mouse’s uterus, and waited about twenty days for a baby mouse to show up. This all sounds complicated, but it’s not. After some training, a college student can do it. It’s such a commodity these days, you can even order a mouse out of a catalog or pay a company to make you one to your specifications.
The baby mice were born perfectly normal, as expected, since the cutting enzyme was switched off at that stage. We called them affectionately “ICE mice,” ICE standing for “Inducible Changes to the Epigenome.” nothing different about these mice until we feed them a low dose of tamoxifen. This is an estrogen blocker that is normally used to treat human cancers, but in this case, we’d engineered the mouse so that tamoxifen would turn on the I-PpoI gene.
The mice might have died. They might have grown tumors. Or they might have been perfectly fine, no worse off than if they’d received a dental X-ray. Nobody had ever done this before in a mouse, so we didn’t know. But if our hypothesis about epigenetic instability and aging was correct, the tamoxifen would work like the potion that Fred and George Weasley used to age themselves in Harry Potter and the Goblet of Fire. And it worked. Like wizardry, it did.
[ Having been prof. myself, the juiciest experiment is when a PHD scholar experiments with many possible outcome, does one correct only by his theory. This is one, demolishing ALL religions and theories of the past.]
Fun Anecdote
To create a mouse to test the information theory, we inserted I-PpoI into a circular DNA molecule called a plasmid, along with all the regulatory DNA elements needed to control the gene, and then inserted that DNA into the genome of a mouse embryonic stem cell line we were culturing in plastic dishes in the lab. We then injected the genetically modified stem cells into a 90-cell mouse embryo called a blastocyst, implanted it into a female mouse’s uterus, and waited about twenty days for a baby mouse to show up. This all sounds complicated, but it’s not. After some training, a college student can do it. It’s such a commodity these days, you can even order a mouse out of a catalog or pay a company to make you one to your specifications.
The baby mice were born perfectly normal, as expected, since the cutting enzyme was switched off at that stage. We called them affectionately “ICE mice,” ICE standing for “Inducible Changes to the Epigenome.” nothing different about these mice until we feed them a low dose of tamoxifen. This is an estrogen blocker that is normally used to treat human cancers, but in this case, we’d engineered the mouse so that tamoxifen would turn on the I-PpoI gene.
The mice might have died. They might have grown tumors. Or they might have been perfectly fine, no worse off than if they’d received a dental X-ray. Nobody had ever done this before in a mouse, so we didn’t know. But if our hypothesis about epigenetic instability and aging was correct, the tamoxifen would work like the potion that Fred and George Weasley used to age themselves in Harry Potter and the Goblet of Fire. And it worked. Like wizardry, it did.
[ Having been prof. myself, the juiciest experiment is when a PHD scholar experiments with many possible outcome, does one correct only by his theory. This is one, demolishing ALL religions and theories of the past.]
Fun Anecdote
[After a few months, David vacations Australia, gets call from excited lab lady] I asked her to text me a photo of the [sick] mouse she was talking about. When the photo came over my phone, I couldn’t help but laugh. “That’s not a sick mouse,” I replied. “That’s an old mouse.” “David,” she said, “I think you’re mistaken. It says here that it’s the sister of these other mice in the cage, and they’re perfectly normal.”
A tamoxifen-triggered ICE mouse at the same age has thinning, graying hair, a bent spine, paper-thin ears, and cloudy eyes. [i.e selective aging can happen. Human id-twins share only 10-25% similarity in fate and age]
[ This one of the tests. Collectively, David meets my standards of Proof. Theory is not important. When docs can, aging will be fixed. But you need to survive till 2030! That it generates trust in Dr. Sinclair advice is crucial - plenty of sub animals who have bad advice based on gym, dead-rishi farts, medicine, experience etc. Apart from incorporation of his ideas in my routine, critical is accuracy of Horvath and epiClock, only way to conduct aging experiments on self! I only accept empirical proof, all based on tomes of dead rishi or soothsayer are stupid! They are belief of others, but never me.]
A tamoxifen-triggered ICE mouse at the same age has thinning, graying hair, a bent spine, paper-thin ears, and cloudy eyes. [i.e selective aging can happen. Human id-twins share only 10-25% similarity in fate and age]
[ This one of the tests. Collectively, David meets my standards of Proof. Theory is not important. When docs can, aging will be fixed. But you need to survive till 2030! That it generates trust in Dr. Sinclair advice is crucial - plenty of sub animals who have bad advice based on gym, dead-rishi farts, medicine, experience etc. Apart from incorporation of his ideas in my routine, critical is accuracy of Horvath and epiClock, only way to conduct aging experiments on self! I only accept empirical proof, all based on tomes of dead rishi or soothsayer are stupid! They are belief of others, but never me.]
Healthy life-extension
Dr. Sinclair extensions (aped by me in reality) are based on evolution. Nothing (big) follows from correct theory of aging but a major idea - we do keep birth-time-record in DNA of the derivation from parents as in womb. THERE IS STILL REMOVING+RENEWING OLD EPI-GENOME! Unless a cool method achieves that by pill course, evolution ideas unlikely to spread as they need fasting (real), exercise and hot/cold. Can a non-sinclair way be found? Is it statistically independent of Sinclair to allow add?
Breakthrough Gene Therapy Clinical Trial is the World's First That Aims to Reverse 20 Years of Aging in Humans
Yes! Explains digilog. Dr. Yamanaka developed 4 factors that remove all epigenome using Cas-9 of CRISPR. NOBEL 2012. But removing all epi-genome reverts the cell back to womb state. Doing it to an organism gives you largest cancer! Amazingly, even Estee-lauder type fashion company keep scientists who applied similar ideas on skin!First hint that body's 'biological age' can be reversed - Nature
Now comes Nature's magic gift to humans - just halving the concentration of 3 factor's selected, is enough to control loss before cancer comes! A Stanford team, led by Tapash Jay Sarkar, Dr. Thomas A. Rando and Vittorio Sebastiano, say their method, designed to reverse these errors and walk back the cells to their youthful state, does indeed restore the cells’ vigor and eliminate signs of aging.They described their technique as “a significant step toward the goal
of reversing cellular aging” and could produce therapies “for aging and
aging-related diseases.” This was 2018. Action has moved to California!
So Boston is Sinclair. Japan is Yamanaka. California is Arun Arya amelioration.
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