The Arya protocol

The protocol is designed to measure the effectiveness of avariety of products/prayers to scientifically measure any improvements evenwhen the benefits are measurable but they have a random sparse distribution. Can aging claims be statistically tested? First prove yourself they can’t then enjoy my claim of yes! It is widely said that age-extension properties of coQ10 are suspected but not proven! Well, either yes or no, with arya-test I can find in 2 years, not the sixty it has been there!

Consider a protocol for improving the age. Or pain management over a number of patients who can reliability measures their pain on a pain-scale, but can suffer from many diseases at the same time, some not even known! Or someone who claims that proper prayer to his deo reduces the impact of earthquakes. One might dismiss such arguments but it is far better there be a scientific proof against. Such an activity provides provable, as opposed to instinctive, arguments against certain cult-extremes of Christianity, in fact any religion.

Because of the general applicability of the protocol, it is separated away, but be confidentially applied and approved by various grant givers.

This is the protocol itself, first stated in generality, and then applied to the proposal. Two running examples are aging and pain management. The claim-argument under examination is that P increases age and Q reduces pain. Our job is define a statistical double blind scheme such that participants age.i for I in 1..n-age is given, and one concludes from that the membership in P scheme that it improved the expected age. Similarly, from j in 1..n-pain, one can conclude the people treated under Q. Note that numbering is not random!

… the test

The useful ness of prayer can be ascertained by image-idol in many places. The argument then becomes magical properties of the idol only. Perhaps challenging prayer is a bad idea.

Aging test

Almost all physicians consider it pseudo-science; I am not one! But respect physicians 100% in acute medicine and only 50% in chronic medicine. There is an explanation in my weltanschauung why the standard dose-response methods fail in chronic diseases.

That applies to diabetes as well, I happen to believe that reducing sugar concentrations with insulin after a certain age replace one poison for another and brings death closer. Fact of immediate benefit from insulin is noticeable, long term evil effects are not! I consider alternative medicine to have valuable suggestions there, particularly of supplements. They should be exposed to the physician who can then indicate contraindication or more common unproved status, unlikely benefit comment. Using the term “complementary medicine” and “arya protocol”, I believe that many complementary medicine procedures can be placed “beyond proved” to “statistically sound” methods, which are NOT known as mechanism of working to enable it for chronic medicine, but enough statistical proof exists against thug-claimers.

I am very interested in state of my parents, especially welfare in the last stage. That being so, I demand methods that

1.       Do not interfere with their medical care

2.       Improve their life

3.       Extend their age by 10 years

4.       Do not open me to thugs. Robust Age Extension has been age-old wish and criminal abuse is the oldest profession (religions are ALWAYS criminal!).

5.       Extensions of normal research, particularly challenge able and discard able. I do not claim infallibility but do claim strong reasonable belief! Challenge on reasoning is accepted when references are common trusted empirical.

With these filters (5 is science, though challenge able versions require science!), I have concluded

1.       Metformin and Niagen should be used and proved for age extension
2.       Reservatrol and other oxidants are considered too delicate for commercial use
3.       Blood transfusion and rapamycin can be tested within the expected 10 year extension
4.       There is certain chance of better life and rare chance to first singularity
5.       The Kurzweil approach to life-extension is fundamentally flawed in absence of known interactions on materials input particularly with questions on purity!
6.       Aging tests can and will be employed on them and my family!

Feeling better

CoQ10 and ECP are in that category. I have started on CoQ10 and can anecdotally reports works very well and has hooked me. Best way is get supply from US and pay 60% taxes of GST and Customs (handled by carrier DHL after several photo updates). ECP machine awaits my doing  outpatient-ECP and then use my somewhat strange beneficial diet (bad if soyabeen-allergic or anti-convinced, japs seem to handle it well).

More tests

Parbiosis with clone? Transfusion grand kids? Crisis of transfusion to ancestors? … I am rearing to become an author and test scientist (Not mad but within ethics).

Aaqgs-aging

http://aaqg-arunarya.blogspot.in/2017/08/aaqgs-aging.html why this link?

What is inevitable aging?

Aging is NOT fighting mortal diseases. It is to lead to indefinite life which is NOT immortality. You have to understand these two statements very well if ever you wish to criticize Aaqgs-aging in sane manner; all other critics are considered stupid henceforth forever ignored mother fuckers. Any one can be aaqgs-purged by developing the argument to violate any of these two; or others.

The single most important concept is self-funded-trajectory. No government should spend money on unseen benefits; however the benefits are those appropriate for a government. In fact, I strongly suggest patent activity for the government, with the benefits going to funders, funding decisions allowing in non-discriminatory buy-ins at various stages and non-discriminatory availability of government patents to all fee-paying users.

By this token, the Nixon war on cancer should not have been launched. NASA would not have been launched either. Rocket science is in fact self-funding trajectory of ICBM development that would leave us about now, while reused first stage would have needed no shuttle and done in 2000’s. Americans would be talking of going to moon now!

The self-funding-trajectory to indefinite-life-research would be fight against cancer. The stupidest thing that can happen to aging development is entry of a government or bigger subhuman – the professional politician. As law has learned the hard way trying to manage Boston mayoralty administration, the professional managers are no better or worse; just a different profession! Best that can be done by politicians is to leave all self-funded-trajectory alone! War-against-cancer and NASA are monuments to government folly.

Good afternoon to all.. today I thank god.. my mother has already been out of therapy.. it has already happened in a room... that big is my God.. ALWAYS TRUST MY LORD.. my creator .. for God nothing is impossible...

That’s you, lady, my parents are in same boat. You pray to non-existent God, I don’t, but get my solace from deep search in medical world, result

http://aaqg-arunarya.blogspot.in/2017/08/more-on-niagen.html

http://aaqg-arunarya.blogspot.in/2017/07/aging-aaquantum-approach.html

and many more. Neither link search, nor write-up are motivated by profit, but love for my parents. Question I ask is, how  to provide pain free years and more 10 yrs. Both possible, try on self-first. My experiences might benefit you too. Mind you, I am NOT a moral person by your experience perhaps, but definitely non-violent and non-criminal.

There is NO empirical solution but calorie restriction. Niagen and metformin imitate them. CoQ10 and ECP improve life. Ten years for me to try rapamycin and blood-transfusion on me. No one gets hurt, even I value my skin a lot.

Perhaps you can meet me next time in Ghaziabad, India.! In any case read and chase-link-read!

Why this link

A reader may wonder at the self-aggrandizement represented by including a link to self in the document. It is not so, the stories are copied verbatim by me to send and also by readers to keep the copy when examined.

But the story might (often) changes after the copied alternative! The latest copy can always be referenced from the link included with the article.

More on Niagen

(1 year later) Nad+ is the goal, NMN is 1step away, avoids NAMPT bottleneck, Niagen is two steps away - A form of Vitamin B3. But NMN is 1.5 times more expensive.

http://aaqg-arunarya.blogspot.in/2017/08/more-on-niagen.html why this link?

This is a patent protected nutraceutical, all its commercial forms are derived from chromadex niagen, and hence quality difference is from additional chemicals and interactions.

Niagen boosts NAD+. What's that?

xidative stress and decreased DNA damage repair in vertebrates increase with age also due to lowered cellular NAD+. NAD+ depletion may play a major role in the aging process at the cellular level by limiting (1) energy production, (2) DNA repair, and (3) genomic signaling. In this study, we hypothesize that it is not NAD+ as a cofactor in redox reactions and coenzyme in metabolic processes that has the ultimate role in aging, but rather the role of NAD+ in cellular signaling when used as substrate for sirtuins (SIRT1-7 in mammals) and PARPs [Poly(ADP-ribose) polymerases]. Both sirtuins and PARPs influence many transcription factors and can affect gene expression. As a signaling molecule, NAD+ is consumed in the reaction donating ADP-ribose and releasing nicotinamide (NAM) as a by-product. It seems that aging at the cellular level is associated with a decline of NAD+ and that NAD+ restoration can reverse phenotypes of aging by inducing cellular repair and stress resistance. Adequate intracellular NAD+ concentrations may be an important longevity assurance factor, while lowered cellular NAD+ concentration may negatively influence the life span.

What is the state of niagen as per FDA?

This 2015 determination is validated just a few days ago.

Why should anyone believe Niagen?

1.     Ultimate proof is empirical, a Japanese study started in 2016, how will anyone prove anti-aging?

2.     Cells have NaDH – NAD+ cycle. One can show empirically that NAD+ level FALL directly vary with age and tiredness correlated with age.

3.     Eating Niagen provably boosts the level of NAD+ by empirical standard measures.

4.     Anecdotally, consumers feel better. A dosage level of 250 mg has been set by informal studies (larger doses do not raise NAD+ SIGNIFICANTLY before fall to 250 mg level).

5.     It is very expensive, far more than coQ10. Cheapest reliable I could find was at amazon,  at Rs. 6913 (when link taken) per month! It is not likely to fall. I see a battle-royal on drug patents by 2020!

Some more references?

1.    Reversing Aging: Not as Crazy as You Think. TIME, 2013 Dec 19

What makes cells age? Wear and tear, yes. But biologically, says, Dr. David Sinclair, professor of genetics at Harvard Medical School, it’s lack of oxygen that signals cells that it’s their time to go. Without oxygen, the energy engines known as the mitochondria become less efficient at turning physiological fuel like glucose into the energy that the cells need to function. Eventually, they shut down…

2.  Niagen Nicotinamide Riboside New NDI Status from FDA

ChromaDex Lead Ingredient NIAGEN® Nicotinamide Riboside Receives New Dietary Ingredient (NDI) Status From the FDA

November 16, 2015

IRVINE, Calif., Nov. 16, 2015 (GLOBE NEWSWIRE) -- ChromaDex Corp. (OTCQX:CDXC), an innovator of proprietary health, wellness and nutritional ingredients that creates science-based solutions for dietary supplement, food and beverage, skin care, sports nutrition, and pharmaceutical products announced today it has received New Dietary Ingredient (NDI) status from the FDA for its patented and proprietary lead ingredient, NIAGEN^® nicotinamide riboside….

3.    Dose-Dependent Elevation of the Blood NAD+ Metabolome by NR

Dose-Dependent Elevation of the Blood NAD Metabolome by NR in Healthy Human Beings: Clinical Efficacy and Novel Diagnostic Biomarkers Abstract Nicotinamide riboside chloride (NR) is in wide use as an orally available NAD precursor vitamin. Here we conducted three experiments to determine the time and dose-dependent effects of NR on blood and liver NAD metabolomes in people and in mice, respectively. We report that human blood cell NAD+ can rise as much as 2.7-fold with a single dose of NR, that NR elevates mouse hepatic NAD+ with distinct and superior kinetics to those of Charles Brenner1 , Samuel AJ Trammell1 , Mark S Schmidt1 , Benjamin J Weidemann1 , Philip Redpath2 , Marie E Migaud2 , Frank Jaksch3 & Ryan W Dellinger3 University of Iowa, USA1; Queens University Belfast, Northern Ireland2 and ChromaDex, Inc, USA3 NAD+ Biosynthesis Study Design Phase: Day: -14 1 2 8 9 15 16 Visit: 1 2 3 4 5 6 7 2 Weeks Screening Treatment Period (3 Weeks) 7 Day Washout 7 Day Washout NAD metabolites undergo circadian oscillation(2,3). To eliminate circadian oscillation as an experimental confounder, we developed an oral gavage protocol in which male C57BL/6 mice were given single oral doses of NR at 185 mg/kg 20’, 1 hr, 2 hr, 4 hr, 6 hr, 8 hr and 12 hr prior to sacrifice, which was always performed at ~ 2 pm. In addition, mice were dosed with equimole amounts of NA, Nam and a control saline solution, and mice were sacrificed at 2 pm without gavage.

supplementationJ Neurosci. 2006 Aug 16;26(33):8484-91 (click to download)

4.   

Nature. 2004;429:771–776. doi: 10.1038/nature02583

Calorie restriction extends lifespan in organisms ranging from yeast to mammals¹. In yeast, the SIR2 gene mediates the life-extending effects of calorie restriction². Here we show that the mammalian SIR2orthologue, Sirt1 (sirtuin 1), activates a critical component of calorie restriction in mammals; that is, fat mobilization in white adipocytes. Upon food withdrawal Sirt1 protein binds to and represses genes controlled by the fat regulator PPAR-γ (peroxisome proliferator-activated receptor-γ), including genes mediating fat storage. Sirt1 represses PPAR-γ by docking with its cofactors NCoR (nuclear receptor co-repressor) and SMRT (silencing mediator of retinoid and thyroid hormone receptors). Mobilization of fatty acids from white adipocytes upon fasting is compromised in Sirt1^+/− mice. Repression of PPAR-γ by Sirt1 is also evident in 3T3-L1 adipocytes, where overexpression of Sirt1 attenuates adipogenesis, and RNA interference of Sirt1 enhances it. In differentiated fat cells, upregulation of Sirt1 triggers lipolysis and loss of fat. As a reduction in fat is sufficient to extend murine lifespan³, our results provide a possible molecular pathway connecting calorie restriction to life extension in mammals.​

5.    Rejuvenation Res. 2016 Mar 16. DOI: 10.1089/rej.2015.1767

Oxidative stress and decreased DNA damage repair in vertebrates increase with age also due to lowered cellular NAD+. NAD+ depletion may play a major role in the aging process at the cellular level by limiting (1) energy production, (2) DNA repair, and (3) genomic signaling. In this study, we hypothesize that it is not NAD+ as a cofactor in redox reactions and coenzyme in metabolic processes that has the ultimate role in aging, but rather the role of NAD+ in cellular signaling when used as substrate for sirtuins (SIRT1-7 in mammals) and PARPs [Poly(ADP-ribose) polymerases]. Both sirtuins and PARPs influence many transcription factors and can affect gene expression. As a signaling molecule, NAD+ is consumed in the reaction donating ADP-ribose and releasing nicotinamide (NAM) as a by-product. It seems that aging at the cellular level is associated with a decline of NAD+ and that NAD+ restoration can reverse phenotypes of aging by inducing cellular repair and stress resistance. Adequate intracellular NAD+ concentrations may be an important longevity assurance factor, while lowered cellular NAD+ concentration may negatively influence the life span.

Arya prediction – 21 century communication

Los Alamos National Laboratory has produced the first known material capable of single-photon emission at room temperature and at telecommunications wavelengths. These carbon nanotube quantum light emitters may be important for optically-based quantum information processing and information security, while also being of significant interest for ultrasensitive sensing, metrology and imaging needs and as photon sources for fundamental advances in quantum optics studies.

Read more at: https://phys.org/news/2017-07-single-photon-emitter-quantum-info-processing.html#jCp

Why century 21?

In optical communication, critical information ranging from a credit card number to national security data is transmitted in streams of laser pulses. However, the information transmitted in this manner can be stolen by splitting out a few photons (the quantum of light) of the laser pulse. This type of eavesdropping could be prevented by encoding bits of information on quantum mechanical states (e.g. polarization state) of single photons. The ability to generate single photons on demand holds the key to realization of such a communication scheme

Read more at: https://phys.org/news/2015-09-nanotubes-path-quantum-technologies.html#jCp

Why the prediction?  

Ideally, a single photon emitter will provide both room-temperature operation and emission at telecom wavelengths, but this has remained an elusive goal. Up to now, materials that could act as single photon emitters in these wavelengths had to be cooled to liquid helium temperatures, rendering them much less useful for ultimate applications or scientific purposes

How done?

Force the nanotube to emit light from a single point along a nanotube, only at a defect site. The key was to limit defect levels to one per tube. One tube, one defect, one photon. . . . By emitting light only one photon at a time, one can then control the photons' quantum properties for storage, manipulation and transmission of information. Wavelength controlled by carrying tube diameter, you can make different frequency elements. Change it dynamically – tunable room temperature single photon emitter is next step. Also doable by dynamic pinching of many tubes in parallel. Hence the prediction.

How built?

This degree of control using diazonium-based chemistry, a process they used to bind an organic molecule to the nanotube's surface to serve as the defect. The diazonium reaction chemistry allowed a controllable introduction of benzene-based defects with reduced sensitivity to natural fluctuations in the surrounding environment. Importantly, the versatility of the diazonium chemistry also permitted the researchers to access the inherent tunability of nanotube emission wavelengths.

Aging, the aaquantum approach

http://aaqg-arunarya.blogspot.in/2017/07/aging-aaquantum-approach.html

If we cured cancer we would add only 3.3 years to an averagelife; solving heart disease gets us an extra four. If we eliminated alldisease, the average life span might extend into the nineties. To live longer,we’d have to slow aging itself.

The solution is NOT just follow the doctors (great acute, poor chronic), all they end up doing is delay death and replace the cause of death! It became heart disease and cancer last century. It will be Parkinson and Alzheimer this century. And mind you, the genetic causes will still lead to variations in life-span, just the valuable gene sets will change!

Aaquantum-I have read lot and believe I am ready with an integrated solution as trial balloon. Not only are ALL religious considered to be criminals, MOST anti-aging eminences are considered to be fools for not understanding the opening paragraph. Ultimately, the only thing that counts is empirical demonstration.

All my recommendations are speculative generalization extrapolations of established experiments to watch and support. It is very important to enlarge the case set of methods to introduce better atoms.

The method I seek is to move to a singularity in 2040’s to thousand year life, to extension using non-biological bodies. This extension is not relevant to first singularity – good luck to those who think otherwise.

The first singularity requires extension in age to between ninety and a hundred for me, doable with existing methods. The point is whether this range is likely and if the awaited singularity occurs.

Approach – Only five things are needed and can be and have been already incorporated or planned. These are niagen, coQ10, metformin, rapamycin and ECP. Beyond that is wittig-like reaction on niagen to make mitoq equivalent. No matter what my genes I think that religious attachment to exercise and blood-rotation will see me through with these. I have talked enough, documentation available on all others but blood-rotation, discussed briefly here, and great detail later. So the two points, beyond rapamycin,  are awaited-singularity and blood-rotation.

Blood-rotation: The experiment that drives this is effect ofsurgical joining of circulatory systems of two mice, one old and one young. The age symptoms of the old mouse disappeared. Scientists have already started to devise human tests in ethical manner (young ages!). What a gold-mine of tech fi stories I can write! The low cost development model does not benefit from centralization.

The rejuvenating effects are seen when lab mice are joined together in a rather gruesome procedure called parabiosis. That involves making cuts in the skin of two animals, then suturing them together at the site of the wound. As the cuts heal, the pair’s blood vessels will grow together and merge. The result is two animals that share a circulatory system, with both hearts pumping both sets of blood around both bodies. Doing this with an old mouse and a young mouse has some spectacular effects. As with humans, old mice have a harder time healing from injuries. But link an old mouse to a young one and it becomes able to repair muscle injuries nearly as well as its younger counterpart. Similar benefits are seen in liver cells and the nervous system. And it works in reverse, too: old blood can have a decrepifying effect on the young. 

Awaited-singularity: Some trees live 4000 years! Whathappens is that stem cells are placed in circulation by roots and reach theparts where cells divide. Not great accuracy is needed – if the division cellis poor, the stem cell progeny lives. It won’t work for humans because the pluripotent stem cell has already specialized. However, iPS cells can be specialized and injected in various organs. One consequence will be fight against cancer! There is hence a well-funded development trajectory.

para added in late aug 2017 - time to celebrate "I told you so!" Transfection is how tree-like life extension will go to defeat cancers.