Saturday, September 4, 2021

Hyperbaric oxygen therapy study 2

After writing: Judged erroneous, not amrit, coz extension of telomeres does not reduce age! BUT Katcher E5, under tests, amazed the greats by actually reducing age, hence real amrit when a product.

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Research Paper Volume 12, Issue 22 pp 22445—22456

Hyperbaric oxygen therapy increases telomere length and decreases immunosenescence in isolated blood cells: a prospective trial

Arun Arya comments are so.

zArun Arya stuff is like this.Introduction

Aging can be characterized by the progressive loss of physiological integrity, resulting in impaired functions and susceptibility for diseases and death. This biological deterioration is considered a major risk factor for cancer, cardiovascular diseases, diabetes and Alzheimer’s disease among others.

That is why it is health span, not life span for me. Unlike eligible patients of the study, cardiovascular diseases, diabetes and Alzheimer’s, osteoarthritis and essential tremors affect me, passed on by my father, affect me. He succumbed at 88. There is no cure for them in mm. He successfully battled the disabilities to end by diet, exercise and mm treatments. His exercise was lifelong yoga. I don’t consider yoga as efficient as long fast energetic walks and gym machines. I ape him for diet and mm treatment (two specialist doctors and earlier advice re cardiovascular). Unlike mother, I consider Ayurveda as iffy treatment, dangerous of all heavy metals. Some parts are very good, but which?

At the cellular level, there are two key hallmarks of the aging process: shortening of telomere length and cellular senescence [1].

Telomeres are tandem nucleotide repeats located at the end of the chromosomes which maintain genomic stability. Telomeres shorten during replication (mitosis) due to the inherent inability to fully replicate the end part of the lagging DNA strand [2]. Telomere length (TL), measuring between 4 to 15 kilobases, gradually shorten by ~20-40 bases per year and is associated with different diseases, low physical performance and cortical thinning of the brain [3–5]. When TL reaches a critical length, cells cannot replicate and progress to senescence or programmed cell death [6]. Goglin et al. demonstrated that adults with shorter TLs have increased mortality rates [7].

Programmed cell death is an important theory, to the extent that there are no evolutionary advantages for survival over reproduction. Our natural advantages arise from slow detiorations of evolutionary advantages. Long life is a certain evolutionary trait. Real aging is a consequence of many genetic factors, two identified so far, the shortening of telomere to Hayflick limit and error correction failure. These are cellular. Others will be identified of organ aging but can be fixed by higher level methods derived from mRNA, Yamanaka factors and blood rejuvenation.

Shortened TLs can be a direct inherited trait, but several environmental factors have also been associated with shortening TL including stress, lack of physical endurance activity, excess body mass index, smoking, chronic inflammation, vitamins deficiency and oxidative stress [2, 8, 9].

Factors identified by black box medicine without identifying the cellular causes are important for life style modifications. Stress is an essential part of computer science profession, all greats have had very poor life-size records. I escaped with TBI after my accident bat 35. It explains to me the several year failure to build unbuggy software, despite it works mostly and only person on earth able to solve multi bit Cocks IBE problem. The lack of physical endurance activity was fixed 10 years ago by 2km walk per day and gym. The excess body mass index problem went away with mostly vegetarian diet. The smoking was ended 30 years ago. The chronic inflammation is not there. A vitamins deficiency can not happen with daily a-z. Any oxidative stress should fix with Liposome NMN and resveratrol.

Cellular senescence is an arrest of the cell cycle which can be caused by telomere shortening [10], as well as other aging associated stimuli independent of TL such as non-telomeric DNA damage [1]. The primary purpose of senescence is to prevent propagation of damaged cells by triggering their elimination via the immune system. The accumulation of senescent cells with aging reflects either an increase in the generation of these cells and/or a decrease in their clearance, which in turn aggravates the damage and contributes to aging [1].

I have strongly asserted that NAD+ incrementation must be tied to senescent cell claring by senolytic like Quertecin. Great if HBOT adds to it.

A growing body of research has found several pharmacological agents that can reduce the telomere shortening rate [11, 12]. Several lifestyle interventions including endurance training, diets and supplements targeting cell metabolism and oxidative stress have reported relatively small effects (2-5%) on TL^3, [2, 8, 9].

Hyperbaric oxygen therapy (HBOT) utilizes 100% oxygen in an environmental pressure higher than one absolute atmospheres (ATA) to enhance the amount of oxygen dissolved in body’s tissues. Repeated intermittent hyperoxic exposures, using certain HBOT protocols, can induce physiological effects which normally occur during hypoxia in a hyperoxic environment, the so called hyperoxic-hypoxic paradox [13–16]. In addition, it was recently demonstrated that HBOT can induce cognitive enhancements in healthy aging adults via mechanisms involving regional changes in cerebral blood flow [17]. On the cellular level, it was demonstrated that HBOT can induce the expression of hypoxia induced factor (HIF), vascular endothelial growth factor (VEGF) and sirtuin (SIRT), stem cell proliferation, mitochondrial biogenesis, angiogenesis and neurogenesis [18]. However, no study to date has examined HBOT’s effects on TL and senescent cell accumulation.

The aim of the current study was to evaluate whether HBOT affects TL and senescence-like T-cells population in aging adults.

Results

Thirty-five individuals were assigned to HBOT. Five patients did not complete baseline assessments and were excluded. All 30 patients who completed baseline evaluations completed the interventions. Due to the low quality of blood samples (low number of cells or technician error), four patients were excluded from the telomere analysis and 10 patients from senescent cell analysis (Figure 1). The baseline characteristics and comparison of the cohorts following exclusion of the patients are provided in Table 1. There were no significant differences between the three groups (Table 1).

Figure 1. Patient flowchart.

Table 1. Baseline characteristics.

		HBOT	Telomere analysis	Senescent analysis	P-value
N		30	25 (83.3%)	20 (66.6%)
Age (years)		68.41±13.2	67.56±14.35	66.70±16.00	0.917
BMI		26.77±3.20	26.89±3.34	27.14±3.81	0.946
Males		16 (53.3%)	13 (52.0%)	10 (50.0%)	0.987
Females		14 (47.7%)	12 (48.0%)	10 (50.0%)	0.987
Complete blood count
	Hemoglobin	6.33±1.25	6.57±1.15	6.58±1.29	0.707
	White blood cells	14.02±1.40	13.92±1.35	13.97±1.49	0.969
	%PBMC	39.96±6.75	39.25±6.64	38.59±6.63	0.774
	Platelets	239.87±1.39	244.08±43.0	254.05±41.4	0.559
Chronic medical conditions
	Atrial fibrillation	4 (13.3%)	4 (16.0%)	2 (10.0%)	0.841
	Hypothyroidism	4 (13.3%)	4 (16.0%)	3 (15.8%)	0.956
	Obstructive sleep apnea	4 (13.3%)	4 (16.0%)	3 (15.0%)	0.961
	Asthma	1 (3.3%)	1 (4.0%)	0	0.680
	BPH	7 (23.3%)	5 (20.0%)	6 (30.0%)	0.733
	GERD	3 (10%)	2 (8.0%)	2 (10.0%)	0.961
	Osteoporosis	5 (16.7%)	5 (20.0%)	4 (20.0%)	0.936
	Rheumatic arthritis	1 (3.3%)	0	1 (5.0%)	0.561
	Osteoarthritis	7 (23.3%)	4 (16.0%)	5 (25.0%)	0.755
	Diabetes mellitus	3 (10%)	3 (12.0%)	2 (10.0%)	0.966
	Hypertension	7 (23.3%)	5 (20.0%)	5 (25.0%)	0.918
	Dyslipidemia	16 (53.3%)	14 (56.0%)	12 (60.0%)	0.897
	Ischemic heart disease	2 (6.7%)	1 (4.0%)	2 (10.0%)	0.725
	History of smoking	10 (33.3%)	8 (32.0%)	7 (35.0%)	0.978
Chronic medications
	Anti-aggregation	8 (26.7%)	6 (24.0%)	5 (25.0%)	0.974
	ACE-Inhibitors/ARB blockers	6 (20%)	6 (24.0%)	6 (30.0%)	0.720
	Beta blockers	5 (16.7%)	5 (20.0%)	3 (15.0%)	0.901
	Calcium blockers	3 (10%)	3 (12.0%)	2 (10.0%)	0.966
	Alpha blockers	7 (23.3%)	5 (20.0%)	6 (30.0%)	0.733
	Diuretics	2 (6.7%)	1 (4.0%)	1 (5.0%)	0.906
	Statins	10 (33.3%)	9 (36.0%)	7 (35.0%)	0.978
	Oral hypoglycemic	1 (3.3%)	1 (4.0%)	1 (5.0%)	0.958
	Bisphosphonates	1 (3.3%)	1 (4.0%)	1 (5.0%)	0.958
	Proton pump inhibitors	3 (10%)	3 (12.0%)	3 (15.0%)	0.726
	Hormones	3 (10%)	3 (12.0%)	2 (10.0%)	0.966
	Benzodiazepines	3 (10%)	2 (8.0%)	1 (5.0%)	0.816
	SSRI	5 (16.7%)	5 (20.0%)	3 (15.0%)	0.990

Telomere length

Compared to the baseline, the T-helper telomere lengths were significantly increased at the 30^th session and post-HBOT by 21.70±40.05 (p=0.042), 23.69%±39.54 (p=0.012) and 29.30±38.51 (p=0.005), respectively (Figure 2). However, repeated measures analysis shows a non-significant trend (F=4.663, p=0.06, Table 2 and Figure 2).

Figure 2. Telomere length changes with HBOT. Mean+SEM *p<0.05, **p<0.01, ***p<0.001.

Table 2. Telomere length and senescent cell changes post-HBOT.

		Absolute changes			Relative changes (%)			Repeated measures F (p)
PBMC	Baseline	30^thSession	60^thSession	Post HBOT	30^th session	60^thsession	Post-HBOT	Repeated measures F (p)
PBMC ((N=25)	2.55±0.53			-0.15±0.40			-4.91±16.70	1.987 (t) 0.09
PBMC (N=20)	2.50±0.53			-0.13±0.31			-4.21±11.99	1.810 (t) 0.07
Relative telomeres length (N=25)
Natural killer	9.27±1.91	11.77±5.14 (0.045)	10.73±2.73 (0.013)	11.75±4.22 (0.06)	25.02±51.42	20.56±33.35	22.16±44.81	0.812 (0.391)
B-cells	8.36±2.02	10.22±3.04 (0.007)	11.23±3.58 (0.0001)	11.17±2.98 (0.007)	25.68±40.42	29.39±23.39	37.63±52.73	7.390 (0.017)
T Helper	8.04±1.82	9.92±3.68 (0.042)	9.63±2.17 (0.012)	10.20±2.77 (0.005)	21.70±40.05	23.69±39.54	29.30±38.51	4.663 (0.063)
T Cytotoxic	8.26±1.54	9.83±4.08 (0.11)	10.08±3.33 (0.019)	10.15±2.74 (0.023)	18.29±45.62	24.13±40.88	19.59±33.98	1.159 (0.310)
Senescent cells (% of T cells) (N=20)
T Helper	10.29±5.42	7.84±7.09 (0.09)	8.51±7.45 (0.20)	6.22±4.88 (<0.0001)	-19.66±80.03	-11.67±94.30	-37.30±33.04	8.548 (0.01)
T Cytotoxic	52.19±21.07	45.53±19.91 (<0.0001)	45.45±18.81 (0.002)	46.59±21.91 (0.0004)	-12.21±8.74	-9.81±9.50	-10.96±12.59	6.916 (0.018)
P-values shown in () compared to baseline.
P-values in bold <0.05.

Compared to baseline, telomere lengths of B cells increased significantly at the 30^th session, 60^th session and post-HBOT by 25.68%±40.42 (p=0.007), 29.39%±23.39 (p=0.0001) and 37.63%±52.73 (p=0.007), respectively (Figure 2). Repeated measures analysis shows a significant within-group effect (F=0.390, p=0.017, Table 2 and Figure 2).

Compared to baseline, natural killer cells telomer lengths significantly increased at the 30^th session (p=0.045) and at the 60^th session by 20.56% ±33.35 (p=0.013). Post-HBOT, telomere lengths increased by 22.16%±44.81 post-HBOT (p=0.06, Table 2 and Figure 2). Repeated measures analysis indicates that there was no additional significant effect after the 30^th session (F=0.812, p=0.391).

Compared to baseline, cytotoxic T-cells had a non-significant increase at the 30^th session by 18.29%±45.62 (p=0.11), followed by a significant increase of 24.13%±40.88 at the 60^th session (p=0.0019) and 19.59%±33.98 post-HBOT (p=0.023). Repeated measures analysis indicates that there was no additional significant effect after the 30^th session (F=1.159, p=0.310, Table 2 and Figure 2).

Excellent for part 1. Clearly will supplement NMN mix in orthogonal ways.

Senescent cells

There was a non-significant decrease in the number of senescent T-helpers at the 30^th session and 60^thsession by -19.66%±80.03 (p=0.09) and -11.67%±94.30 (p=0.20) respectively. However, there was a significant drop in the number of senescent T helpers by -37.30%±33.04 post-HBOT (P<0.0001, Figure 3). Repeated measures analysis showed a significant continuous effect even after the 30^th session, with a within-group effect (F=8.547, p=0.01, Table 2 and Figure 3).

Figure 3. Senescent cell changes with HBOT. Mean+SEM *p<0.05, **p<0.01, ***p<0.001.

T-cytotoxic senescent cell percentages decreased significantly by -12.21%±8.74 (P<0.0001) at the 30^th HBOT session, -9.81%±9.50 at the 60^th HBOT session (0.002) and -10.96%±12.59 (p=0.0004) post-HBOT (Table 2and Figure 3). Repeated measures analysis shows a significant continuous effect even after the 30^th session, with a within-group effect (F=6.916, p=0.018, Table 2).

Excellent for part 2. Clearly will supplement Quertecin in orthogonal ways.

HIF-1alpha

HIF-1alpha levels were increased from 10.54±3.39 to 19.71±3.39 at the 60^th session (p=0.006) where 2 weeks post HBOT levels of 16.81±7.65 were not significantly different from baseline (p=0.16).

Excellent for part HIF-1alpha. Has no evil effect.

Wednesday, September 1, 2021

Study of the AVIV paper

After writing: Judged erroneous, not amrit, coz extension of telomeres does not reduce age! BUT Katcher E5, under tests, amazed the greats by actually reducing age, hence real amrit when a product.

Aging is best defined as a biological process with aging hallmarks. The Hallmarks of Aging. Of 9, they focus on 2.

Repeated intermittent hyperoxic exposures, using certain hyperbaric oxygen therapy (HBOT) protocols, can induce regenerative effects which normally occur during hypoxia. The aim of the current study was to evaluate whether HBOT affects TL and senescent cell concentrations in a normal, non-pathological, aging adult population.

Their magic is several hyperoxic and hypoxic cycles per session Rejuvenation occurs in hypoxia part of the cycle. This is like intermittent muscle strengthening. They do not disclose how many cycles per session or details of oxygen waveform. Reverse engineering may have to be done. Mice models will be needed for these engineering details. I assume that 12 week, 5 times 2 hour sessions per week can be copied at this point. Triangular face mask type HBOT allows chair sitting and can be modified to sit on exercise bike!

Methods: Thirty-five healthy independently living adults, aged 64 and older, were enrolled to receive 60 daily HBOT exposures. Whole blood samples were collected at baseline, at the 30^thand 60^th session, and 1-2 weeks following the last HBOT session. Peripheral blood mononuclear cells (PBMCs) telomeres length and senescence were assessed.

We can enrol Indians from low classes for three month. Wages can be paid, boon for retirees at 65. The aging benefits are there! In fact, many people will pay to be in the study. One machine can be used by 12 people.

Results: Telomeres length of T helper, T cytotoxic, natural killer and B cells increased significantly by over 20% following HBOT. The most significant change was noticed in B cells which increased at the 30^th session, 60^th session and post HBOT by 25.68%±40.42 (p=0.007), 29.39%±23.39 (p=0.0001) and 37.63%±52.73 (p=0.007), respectively.

It is medicine saying that if the immune system can be saved from time, a person can live for 1000 years! All the benefits above are in the immune system in human blood.

There was a significant decrease in the number of senescent T helpers by -37.30%±33.04 post-HBOT (P<0.0001). T-cytotoxic senescent cell percentages decreased significantly by -10.96%±12.59 (p=0.0004) post-HBOT.

Fixing the immune system is by lengthening the telomere and by garbage-collecting the senescent cells. Both are being done! Low cost small delta variations can be tried in India, and only here!

In conclusion, the study indicates that HBOT may induce significant senolytic effects including significantly increasing telomere length and clearance of senescent cells in the aging populations

Tuesday, August 31, 2021

Welcome Singularity

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The golden paper

After writing: Judged erroneous, not amrit, coz extension of telomeres does not reduce age! BUT Katcher E5, under tests, amazed the greats by actually reducing age, hence real amrit when a product.

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In Hindi, they call it amrit. In 2021, knowledgeable know it as singularity. It came a few days ago, unheralded. All alive and rich today can live forever, and die only by accident or malice. After they get smart! Historically inclined may call it successful end of Gilgamesh quest after 4000 years.

Science recommendations play into the common narrative that science is an objective means of identifying a single truth. On Aug 27, 2021, Singularity arrived unheralded. Medical means exist for extending human health span to 2050 for the rich who can extend life in 2050 if you can afford Israeli AVIV. There are no commercial disclaimers.

Aging undo has been the most crucial AND fraud-prone demand of human for last 4000 years of recorded history and even mummy discoveries. A tv channel of repute broke the story 2 days earlier THAT An Israel company had provably done aging undo, measured by telomere lengthening of 20-40 percent in age of 75 of 35 people free from diseases of the aged, agreed to by wonderful feeling in lucky patients, had written a research paper just accepted by a top peer reviewed journal Aging and published.

This is an amazing paper, studies here.

There was little reporting of this maximally interesting story in print media. It is probably an interesting medical story, nothing more. Like statin use, I started in 1998 for heart, 5 years later, it was prescribed even to US dogs, It hit India in big way in 2008. I got my medicine cheap being on wife’s family policy. Then the patent expired. Companies worked on developments, storvas or atorvastatin for me. This is 2021. People should start early, as the external looks do not become those of 25 years yet. It may never happen,

What is aging? Can be captured in hallmarks. Simply, a biological process with these hallmarks. Definition so can focus on alleviation. These are sufficient to cover all the aging disabilities and accepted by all doctors.

Armed with just TV story, I applied my aging readings to chase the story in internet. The medical team claiming to do so is AVIV company of Israel, also in Florida in USA and UAE in Asia They use HBOT to achieve it. Their protocol needs 5 daily sessions of 2 hour each for 12 weeks. Difference to normal protocol is several on-off convention periods. Blind/approximate work can be life threatening. Exact details are not disclosed. The patients are exposed to important psychological training and body workout.

The exact method is not known. Of the many methods for healthy life extension, I do not believe this can be repeated indefinitely, essential but no promise of indefinite life. It happened too quickly but I do believe in print that it is the singularity. It will help me and my patients to avail themselves of non-cutting-edge technologies to introduce some experience in methods. There are two early provable measurable benefits – lengthening of telomere and garbage-collection of senescent cells.

How do I know? Dr. Aubrey de Grey (saint of Aging Undo) appreciates the method and considers it plausible. That plus acceptance in a top peer viewed journal removes my skepticism. HBOT machines were investigated by me as a useful patient diversion when doing ECP, reason of why they exist and have a market – they are famed for glowing skin and used by aging film stars and high-end ladies. Their usefulness in Aging undo is an added benefit that makes investment in them a no-brainer, for ne who intends to recreate the protocol with Modi or Yogi, in case AVIV refuses a joint venture in India, like UAE and Florida. India can afford to benefit upper classes throughout Asia and Africa. Work will also benefit middle and lower classes in 5years. But this essay is preliminary to a business plan.

A business cannot be started unless there is technology, exploratory and tracking companies only make sense by already established great using Government funding, Consider the difficulty in setting up clinical test for metformin! No one has found a way yet of advocating that as a way to damage ISIS, Alqaida or Taliban! Basic to my lament is Einstein urging Roosevelt to set up and fund LANL, and continued funding given German effort in WW2. No credible enmity to Soviet Union, now reduced to Russia. But then China is there.

Israeli know this protocol can not be kept confidential for long. India is a natural country to do joint ventures with, doing with each state separately makes sense – miss one and you have a competitor soon!

Copies of the essay for Yogi, Modi, Bihar and via Baldev to Joey. Also to AIIMS Delhi and Rishicase, medical college in M.Nagar and Meerut, all for a possible PP model funded. Ramdev and other Godmen. Healthy life extension Institute in India. Certainly, the known great doctors. People recommended by my batch mates.

My recommended method changes some. A triangular face mask machine can be bought within 10 lakh, and HBOT machines can be rented. All that means Liposome NMN and Liposome resveratrol are likely supplements. They can be supplanted with some Doctor suggested HBOT protocol. 12 weeks later, I will test myself. Telomere lengtj will be measured at start and end. May keep the numbers to self, maybe. Subjectively measure mood and feelings. Only on significant improvements will I suggest to mother. Else vary protocol and try again, 4 times. Buying makes sense for me. Not just mother, but sisters and husbands all can use my HBOT on success. Only on success will proceed for 12 weeks without NMN.

The telomere-based protocol

I deliberately suppressed this method because people I consider frauds were using this method and Dr. Sinclair had another very effective theory of aging the never mentions Telomere. Even if there is no change in mood, only telomere length benefit, all other methods will benefit. Now I consider telomere-based aging to be orthogonal to Dr. Sinclair and equally valid but distinct. Mind you, it makes sense to apply distinct methods in series and not in parallel. Also, I will be a guinea-pig breaking uncharted grounds as I have aging disabilities. Surprisingly, my TBI may benefit too.

To repeat, only method known to increase healthy age of vertebrates is calorie restriction. The benefits can be expanded and improved by analogous chemicals. Point is that benefits vanish if senescent cells intervene. Senolytics fight that. Cells have reducing telomeres for a very simple evolutionary reason; fixed size telomere are of cancer cells! The gene for elongation is part of DNA, in fact cancer-s-cells discover its use and make themselves infinite lived cancer cells. Best you can do is repeated elongation and natural shortening. How many times? No one knows, don’t assume indefinite life!

A Telomere is the end of DNA in the nucleus. Every division of cells cause daughter cells with shortened telomere. When they become too small, the Hayflick limit is reached and the cell becomes senescent. This limit is part of aging. Remember that NAD+ troubles senescent cells who emit SASP to reduce it. NAD+ increment using NMN and Resveratrol must be tied to senescent cells garbage collection to reduce SASP. HBOT protocol does that with 10-40 percent efficiency. Quertecin does that, I hope to improve on that.

So, the telomere method is a clean add. Given my description of journalist as a proven failure of cleverness, I do not consider the method to be repeatable forever. It is new and can be repeated once in 20 years, it is not amrit, just booster-amrit to wonders of 2050.

HBOT is simple to administer, limited expense, if you know what to do! Quite independent of executives, failure to help wider use of AVIV style technology will hurt the company, avoidable with government joint ventures in capable regions like India. Get ready for medical tourism, particularly Delhi based NCR region.

Concentrations of disparate sciences is excellent for ground breaking research. Through discussion, other researchers ask questions derived from their curiosity, taking the research in a new direction, landing on a very interesting question, which has an even more interesting answer. Computer science and biology is so. It created SENS and me.

In conclusion, their study [paper linked] indicates that HBOT may significantly increase telomere length and do clearance of senescent cells in the aging populations.

Others (so few!)

The Beacons of Light ~ Welcome to the Singularity

The Observer: Welcome to the Singularity

Thursday, July 22, 2021

Third AstraZenica dose, works and when

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I only care for empirical experimental proof whenever theoretical derivation might fail. This is true for the third dose. It is theoretically likely that 3^rd dose will improve immunity but

1. will it empirically?

2. who should get it?

3. expected consequences of not doing it?

4 What benefits?

5 When is vaccine best?

Answers follow, only collected by me for this research, none mine original research.

1. will it empirically?

Yes!

A third dose of the AstraZeneca vaccine is found to boost immune response.

Third dose of AstraZeneca COVID-19 vaccine boosts immune response, Oxford study finds.

2. who should get it

All doctors in front line. All immunodeficient like me from long stay outside the country in USA, very concerned with cleanliness and hence Covid-19 compromised by hygiene theory.

Very Important- I just preceded Dr. Guleria, director AIIMS. He wants you to get 3rd dose, calls it booster dose. He never limited it, I agree!

3. expected consequences of not doing it?

Get Covid-19, likely non-fatal, as no repeated infection like front line doctors. So concerned that I instructed no hospital reporting unless urgent, treat with oxygen generator at home!

4 What benefits?

Escape even mild infection as further compromised by diabetes and hence looking at fungus infect5ions post recovery. Not so conservative to be anti-vaccine, IE be brain-compromised too.

5 When?

UK work says best gap for second has a sweet spot after 6 weeks. So also third after 2^nd,but 6 months. Works out to Nov 1. Time enough to more empirical data and find a station.

6 what vaccine is best?

Theoretical arguments say AstraZeneca. No empirical data yet on another kind despite ongoing trials.

7 Postscript Sept 2, 2021

Americans strongly urge and vaccinate free with booster dose of their mRNA vaccines. so does UK with Astragenica, It is liberal-style mandated.